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Western blotting pattern of Myc-P2Y4 receptor. SH-SY5Y cells transiently transfected with Myc-vector or Myc-P2Y4 were lysed and immunoprecipitated with anti-c-Myc-conjugated agarose. Immunoadsorbents were run on 10% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), blotted and probed with anti-Myc (9E10) antibody. Protein bands were detected with secondary anti-mouse antiserum by electrogenerated chemiluminescence (ECL)
