Figure 5.
Homo-dimerisation of tagged-P2Y4 receptor. SH-SY5Y cell were transiently transfected with Myc-P2Y4, FLAG-P2Y4 or a combination of both tagged receptors. Solubilised proteins were immunoprecipitated with the anti-FLAG resin and detected by Western blot with the anti-Myc antibody. Protein bands were detected with secondary anti-mouse antiserum by electrogenerated chemiluminescence. The first lane (in vitro mix) derives from cells expressing individual tagged receptors that were mixed during lysis and then immunoprecipitated and detected at the above-mentioned conditions