Figure 2.

CLT inhibits formation of polyribosomes. Exponentially growing 3T3 cells were challenged with CLT (10 μM, solid line) or vehicle (broken line) for 30 min. Extracts were prepared and equal OD at 260-nm units were separated by sucrose density gradient centrifugation and polysome profile of the gradients were obtained. The position of 80S, 60S, and 40S material is indicated by arrows.