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CLT inhibits G1/S transition. (A) Quiescent 3T3 cells were stimulated with bFGF (5 ng/ml). Progression into S phase was monitored by measuring [3H]thymidine incorporation at the indicated times after bFGF stimulation. (B) To determine the CLT-sensitive period, bFGF-stimulated cells were challenged with 10 μM CLT at the indicated times after bFGF addition and pulsed for 2 hr with [3H]thymidine added 14 h after bFGF stimulation.
