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Forced expression of cyclin D1 confers resistance to CLT (A); and CLT does not inhibit expression of cyclin D1 in cyclin D1-, PKR-K296-, or eIF2α-51A-transfected cells. Exponentially growing cells were challenged with or without CLT (2.5 μM) for 8 h. Cells were harvested and expression of cyclin D1 and β-actin was determined by immunoblotting.
