Fig. 2.

Enhancement of fluorescence by SIF. Myofibrils (1 mg/mL) labeled with 0.1 μM Rh-phalloidin were placed on a glass coverslip (top left) and on a coverslip coated with SIF (top right). The spectra were measured at a 45° angle in a Varian Eclipse spectrofluorometer. The vertical scale is in arbitrary units (a.u.). The spectrum of SIF in the absence of muscle is less than 10 a.u. at all wavelengths.