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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: Cell Calcium. 2007 Apr 11;42(6):590–605. doi: 10.1016/j.ceca.2007.02.003

Fig. 3.

Fig. 3

SCFA-induced modulation of PMCA expression in various gastric/colon cancer cell lines: time course.

KATO-III (A), DLD-1 (B) and LS-174T (C) cells were cultured in the presence of 3 mM Na+-valerate or Na+-butyrate, as indicated on the panels. Total cellular lysates were prepared from all treatments at the indicated time points and equal amounts of cellular proteins (from 20 to 30 μg/lane, depending on the cell lines and the antibody used for immunostaining) were analyzed for overall PMCA (5F10), PMCA4b (JA3) and for Na+/K+-ATPase expressions. Induction of PMCA4b expression could be detected as early as day 0.5-1, and reached a plateau-phase after 1-2 days of SCFA-treatment in LS-174T cells, and after 4-5 days of SCFA-treatment in KATO-III and DLD-1 cells.