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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: Cell Calcium. 2007 Apr 11;42(6):590–605. doi: 10.1016/j.ceca.2007.02.003

Fig. 4.

Fig. 4

Up-regulated PMCA4b mRNA expression in SCFA-treated KATO-III and DLD-1 cells.

KATO-III gastric (A) and DLD-1 colon (B) cancer cells were treated with 3 mM Na+-valerate or 3 mM Na+-butyrate for 4 or 5 days, respectively. Untreated cells were used as controls. Real-time PCR analysis was conducted by using DNA templates obtained following reverse transcription of mRNAs from SCFA-treated and untreated cells, and PMCA4b-specific oligonucleotide primers. As internal control, GAPDH was also amplified from the same RNA preparations. Normalized PMCA4b mRNA/GAPDH mRNA ratios were calculated by using crossing point values and separate calibration curves for the two amplicons.

The bars in panels (A) and (B) represent the means ± S.D. of three independent determinations of a representative experiment. Statistical significance is denoted by **p<0.01.

The SCFA-induced differentiation resulted in strong induction of PMCA4b mRNA expression in both cell lines.