Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Nov 26;3(7):486–492. doi: 10.7150/ijbs.3.486

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

PMC Copyright notice

Further verification of the effect of BRCA1 knockdown on TIMP1 transcription. (A-B). Transient knockdown of BRCA1 using BRCA1-specific siRNA oligo (DO4 in A and DO7 in B). Mock transfection and a luciferase-specific siRNA oligo were included as controls. siRNA oligos were transfected into EGFP(E) or COBRA1(-C) stable knockdown T47D cells. The immunoblots in Fig. 4A show the extent of BRCA1 depletion in the transient transfection experiment. (B). Control or BRCA1/COBRA1 knockdown cells were starved in medium containing charcoal-stripped serum. Vehicle or estradiol (E2; 10nM final concentration) was added for 24 hr, and RNA was harvested for qRT-PCR analysis of TIMP1 mRNA abundance.