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. Author manuscript; available in PMC: 2007 Nov 28.
Published in final edited form as: Dev Biol. 2007 Mar 3;305(2):551–563. doi: 10.1016/j.ydbio.2007.02.040

Figure 1.

Figure 1

puf-9 depletion enhances the lethal bursting and heterochronic phenotypes of let-7(ts) mutants. (A) let-7(n2853ts) animals exposed to puf-9(RNAi) show an increase in the number of animals that die due to bursting at the adult stage (n=533) compared to let-7(n2853ts) animals grown on a control mock RNAi (n=1114). puf-9(ok1136) mutants (n=746) and wild type animals on puf-9(RNAi) (n=483) showed similar survival rates as let-7(n2853ts) animals on mock RNAi. 100% survival rate was seen in wild type animals grown on mock RNAi (n=652). (B) A cartoon of the intron/exon structure of the puf-9 gene (contained on the W06B11.2 cosmid). The relative position of the region deleted in the puf-9(ok1136) allele is boxed and shaded. The Pum-HD region is indicated within the ok1136 deletion region. The puf-9(ok1136) deletion removes 1581 nt beginning 154 nt into exon 4 and ending 77 nt into exon 8 (nt 5727 to 7309 in the W06B11.2 cosmid sequence). This deletion ends 632 nt upstream of the predicted stop codon and causes a frame-shift to create a premature stop codon 29 nt downstream of the deletion break. (C-E) Depletion of let-7 and puf-9 gene products results in a similar vulval bursting phenotype. Nomarski DIC images of a wild type adult (C, vulva is marked with an arrow), an adult stage let-7(n2853ts) animal with a burst vulva (D), and a puf-9(ok1136) adult animal showing a bursting phenotype similar to let-7(lf) mutants (E). (F) puf-9(RNAi) enhances the lack of adult alae phenotype of let-7(lf) mutants. When grown on puf-9(RNAi), less than half of the let-7(n2853ts) animals displayed any alae at the young adult stage (n=40) compared to wild type animals exposed to puf-9(RNAi) (n=27) or let-7(n2853ts) mutants on mock RNAi (n=61).