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. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: J Neuroimmunol. 2007 Sep 4;190(1-2):139–145. doi: 10.1016/j.jneuroim.2007.07.016

Figure 5.

Figure 5

Degradation of MBP by calpain released from activated human MBP-specific T cells. Incubation conditions are described in text. The activated MBP-specific T cells were incubated in the presence of exogenous MBP and with other factors as follows: lane 1, activated MBP-specific T cells + MBP + EGTA (4 h); lane 2, as lane 1 with Ca2+ for 1h but without EGTA; lane 3, as lane 2 for 4 h; lane 4, as lane 3 + EGTA for 4 h; lanes 5–8: unactivated MBP-specific T cells in the presence of exogenous MBP and other factors. Conditions for incubation in lanes 5–8 are the same as lanes 1–4. The MBP degradation was analyzed by SDS-PAGE and immunoblotting.