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. 2007 Jan 6;3(1-2):39–51. doi: 10.1007/s11302-006-9035-x

Table 1.

Summary of extracellular nucleotide regulation of ROS production by immune and tumor cells

Cell type Treatments* Nucleotide effect on ROS production* Receptors proposed Method for measuring ROS production References
Human neutrophils fMLP + ATP, ADP, or AMP ATP or ADP: ↑ Not discussed SOD-sensitive reduction of ferricytochrome c [68]
AMP: ↑
Immune complex + ATP, ADP, or AMP ATP, ADP, or AMP: ↑
Rat peritoneal neutrophils fMLP + ATP, ADP, or AMP ATP or ADP: ↑ Not discussed Ferrithiocyanate formation ± ferricytochrome c ± SOD [69]
Immune complex + ATP, ADP, or AMP ATP, ADP, or AMP: ↑
Human or rat blood neutrophils fMLP or immune complex + ATP ATP: ↑
Human neutrophils fMLP + ATP, UTP, or ITP ATP, UTP, or ITP: ↑ None identified Ferricytochrome c reduction assay [70]
Human HL 60 cells (promyelocytic leukemia cells) ATP, ADP, dATP, UTP, dUTP, UDP, ITP, CTP, or TTP ATP or UTP: ↑ P2 receptors SOD-sensitive reduction of ferricytochrome c [72]
Human neutrophils ATP ATP: ↑ Not discussed Ferricytochrome c reduction assay [71]
Rat alveolar macrophages ATP, ADP, AMP, or ATPγS ATP, ADP, or ATPγS: ↑ 2 or more P2 receptor classes SOD-sensitive reduction of ferricytochrome c [74]
Human eosinophils ATP, ATPγS, 2-MeS-ATP, UTP, GTP, ADP, BzATP, or CTP ATP, ATPγS, 2-MeS-ATP, UTP, GTP, or BzATP: ↑ P2Y and P2X receptors Lucigenin-dependent chemi-luminescence [75, 76]
oATP or KN62: ↑
Human neutrophils and promyelocytes (HL-60 cells) BzATP BzATP: ↑ P2X7 DCFDA fluorescence (intracellular ROS) [73]
Human DU145 cells (prostate cancer) ATP, ADP, UTP, or 2-MeS-ATP ATP, ADP, UTP, or 2-MeS-ATP: ↑ P2 receptors DCFDA fluorescence (intracellular ROS) [21]
Rat mesangial cells BzATP BzATP: ↑ P2X7 DCFDA fluorescence (intracellular ROS) [77]
Rat microglia BzATP or ATP BzATP or ATP: ↑ P2X7 SOD-sensitive reduction of ferricytochrome c; tetrazolium dye reduction; translocation of p67phox (NADPH oxidase subunit) [78]
Human ARO cells (thyroid cancer) ATP ATP: ↑ P2Y DCFDA fluorescence (intracellular ROS) [20]
Murine RAW 264.7 macrophages BzATP, ATP, UTP, or αβ-Methylene-ATP BzATP or ATP: ↑ P2X7 DCFDA fluorescence (intracellular ROS) [79]

* The upward arrow (↑ indicates that the specified treatment enhanced the indicated parameter (ROS production), whereas the downward arrow (↑ designates that the specified treatment or inhibitor (e.g., oATP or KN-62) attenuated ROS production. SOD superoxide dismutase, DCFDA the intracellular ROS-reactive indicator dye- 2–7–dichlorodihydrofluorescein diacetate.