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. Author manuscript; available in PMC: 2007 Nov 28.
Published in final edited form as: J Biol Chem. 2007 Jul 5;282(35):25278–25289. doi: 10.1074/jbc.M611206200

FIGURE 2. iPLA2β antisense oligonucleotide down-regulates iPLA2β protein and inhibits Ang II-induced RGS2 mRNA up-regulation in VSMC.

FIGURE 2

Cells were transfected with 2 μg of iPLA2β antisense or sense oligonucleotide. The cells were cultured in serum-free medium for 48 h for quiescence, and then stimulated with 100 nm Ang II for 1 h. The iPLA2β and cPLA2 proteins were determined by Western blot using anti-iPLA2β antibody (Santa Cruz) and anti-cPLA2 antibody, respectively. RGS2 mRNA was determined by real-time PCR. A, a representative iPLA2β and cPLA2 Western blot. B, summary of the Western blot shown in A. C, antisense, not sense, inhibits RGS2 mRNA expression. The summary data are from at least three time independent experiments. ***, p < 0.001 versus the basal sample (non-transfected cells); ##, p < 0.01 versus the basal sample (without Ang II stimulation); *, p < 0.05 versus Ang II stimulation alone.