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. Author manuscript; available in PMC: 2008 Nov 16.
Published in final edited form as: Biochem Biophys Res Commun. 2007 May 30;363(2):247–252. doi: 10.1016/j.bbrc.2007.05.150

Figure 2. Reversal of inhibited collagen synthesis and proliferation in SCs cocultured with MSCs by neutralizing IL-10 and TNF-α and reversal of induced apoptosis by neutralizing HGF.

Figure 2

Activated stellate cells were cultured with MSCs separated by Transwell membranes at a fixed ratio of 1:1. PIP-secretion with increasing concentrations of neutralizing antibody for IL-10 (A), TNF-α (B) or TNF-α with a fixed concentration of anti-IL-10 at 1500 ng/ml (C) was measured by ELISA. BrdU staining was measured by flow cytometry with increasing concentrations of neutralizing antibody for IL-10 (D), TNF-α (E) or TNF-α with a fixed concentration of anti-IL-10 at 1500 ng/ml (F). Annexin-V reactivity was analyzed in rat SCs by flow cytometry at increasing concentrations of neutralizing anti-HGF (G). Data represent the mean of two experiments performed in triplicate. Error bars are standard deviation. *p<.05, **p<.01 compared to SCs alone (MSC:SC = 0).