Table 2.
Sequence changes in the coding region of the hprt gene in RAW264.7 cells stimulated to produce NO
| Type of mutation | Number of mutants
|
|||
|---|---|---|---|---|
| No stimulation | IFN-γ, 23 days | LPS + IFN-γ, 4 days | LPS + IFN-γ + NMA, 4 days | |
| Base substitution | ||||
| GC to AT | 3 | 1 | 0 | 1 |
| TA | 3 | 3 | 2 | 0 |
| CG | 3 | 0 | 3 | 0 |
| AT to GC | 4 | 3 | 0 | 1 |
| CG | 5 | 5 | 3 | 3 |
| TA | 1 | 5 | 1 | 1 |
| Total base substitutions | 19 | 17 | 9 | 6 |
| Insertion | ||||
| Small (<8 bp) | 0 | 1 | 1 | 0 |
| Large (+ exons 4-6) | 0 | 1 | 0 | 0 |
| Insertion/deletion | 0 | 1 | 0 | 0 |
| Total insertions | 0 | 3 | 1 | 0 |
| Deletion | ||||
| 1 bp | 0 | 3 | 2 | 0 |
| Others (<72 bp) | 0 | 4 | 1 | 0 |
| Total deletions | 0 | 7 | 3 | 0 |
| Single exon deletion | ||||
| −Exon 2 | 2 | 2 | 3 | 2 |
| −Exon 4 | 2 | 5 | 1 | 1 |
| −Exon 5 | 2 | 7 | 1 | 1 |
| −Exon 6 | 1 | 1 | 2 | 0 |
| −Exon 7 | 1 | 2 | 0 | 0 |
| −Exon 8 | 0 | 0 | 0 | 1 |
| Total single exon deletions | 8 | 17 | 7 | 5 |
| Multiple exon deletion | ||||
| −Exon 2–3 | 0 | 0 | 3 | 1 |
| −Exon 2–4 | 0 | 1 | 0 | 0 |
| −Exon 3–4 | 0 | 1 | 0 | 0 |
| −Exon 4–5 | 0 | 0 | 2 | 0 |
| −Exon 5–6 | 0 | 0 | 0 | 1 |
| −Exon 4–6 | 0 | 0 | 0 | 1 |
| −Exon 2–7 | 0 | 1 | 0 | 0 |
| Total multiple exon deletions | 0 | 3 | 5 | 3 |