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. 1998 Jul 7;95(14):8304–8309. doi: 10.1073/pnas.95.14.8304

Figure 1.

Figure 1

RT-PCR experimental design. BSMV RNAs (α, β, and γ), and MStV genomic (vRNA 4) and mRNA 4 are indicated. Open boxes indicate ORFs, and letters inside boxes indicate the respective encoded proteins. The 5′-terminal nucleotide sequences for the MStV RNAs are shown below the vRNA 4 and mRNA 4. Arrows show the RT-PCR strategy used to detect BSMV/MStV chimeric RNAs containing BSMV 5′-terminal nucleotides (indicated at bottom). cDNA was synthesized using the MStV primer NCP (see Table 1). PCR was performed using a 5′ BSMV and 3′ MStV primer.