Table 1.

Primers used for RT-PCR

Virus RNA*	cDNA primer†	cDNA product‡	5′ PCR primer†	3′ PCR primer†	PCR product§
BSMVα	BSMV7α	211	5′BSMV1α	3′BSMV4α	180
	TACGTGCGAGCACAGTACGT		TTGGATCCCCCCCCGTATGTAAGT	GCGAATTCGGAAGCTGTGCTAATC
BSMVβ	BSMV8β	189	5′BSMV2β	3′BSMV5β	149
	CCCACCAATCGTCAAATACT		TTGGATCCCCCCCCGTAAAAGAAA	GCGAATTCGATGTAGTGTCCTCC
BSMVγ	BSMV9γ	441	5′BSMV3γ	3′BSMV6γ	137
	ATGTCCCGGTAAAGACCACG		TTGGATCCCCCCCCGTATAGCTTG	GCGAATTCTGCTACTACAGTCAC
MStV RNA 4	MStV50	173	5′MStV-CON	3′NCP	121
	CAGATCTAGAAGAGTGATAT		TTGGATCCCCCCCCACACAAAGTC	CAGGCCTGTAATAGGGCCAA

^*Indicates the virus RNA. 

^†Boldface, italicized letters indicate nucleotides that are complementary (for the cDNA and 3′ primers) or identical (the 5′ primer) to the respective virus RNA sequence. Letters not appearing as boldface and italicized were added to primers to aid cloning. 

^‡Indicates expected size of cDNA product, in nucleotides. 

^§Indicates expected size of the RT-PCR product, in nucleotides.