Figure 3.

(A) Picture of a representative EMSA showing that ApCREB1 is a component of retarded band 1. When preincubated with an antibody that specifically recognizes ApCREB1, pedal extracts give rise to a new supershifted band (arrowhead) concurrent with a reduction in the intensity of retarded band 1. Similar results were obtained with each of the two different ApCREB1 antibodies utilized. (B) Picture of representative Western blots using an antibody that recognizes ApCREB2. An increase in ApCREB2 immunoreactivity can be seen in the 24-hr but not the 3-hr postinjury samples. No specific immunoreactivity was detected when the primary antibody was eliminated from the incubation mixture. (C) Summary figure showing that axonal injury significantly increases the immunoreactivity of ApCREB2 by 24 hr postinjury. The data are presented as the mean ± SEM. ∗, P < 0.05.