Figure 6.

Sequestration of CRE-binding proteins causes increased number and length of axon collaterals in regenerating motor neurons. Neurolucida traces of regenerating axons from control (A) and CRE sequence-containing, plasmid-injected (B) neurons. Collaterals can be seen emerging from the regenerating axon (e.g., open arrowhead). In the CRE sequence-containing, plasmid-injected cells, collaterals emerged not only from the tip, but also from the body of the main axon. Some collaterals (solid arrowhead) emerge from the main axon outside the shown field of view. (C) Summary figure showing the average number of collaterals in control and TRE sequence- and CRE sequence-containing, plasmid-injected neurons. The data are presented as the mean ± SEM. ∗, P < 0.05. (D) Summary figure showing the average length of axon collaterals in the control and TRE sequence- and CRE sequence-containing, plasmid-injected neurons. The data are presented as the mean ± SEM. ∗, P < 0.05.