Figure 2.

Neurabin interacts with p70^S6k both in vitro and in vivo. (a) In vitro binding of myc- p70^S6k from transfected HEK293 cells to a GST or GST-neurabin (amino acids 486–751) column. Salt concentrations of washes are indicated. (b) Coimmunoprecipitation of myc-neurabin (amino acids 486–751) with HA- p70^S6k but not HA-AKT (Upper). Equal transfection of HA- p70^S6k and HA-AKT is confirmed by Western blot (Lower). Salt concentrations of washes are indicated. (c) Interaction of p70^S6k with neurabin is phosphorylation-independent. Coimmunoprecipitation of neurabin and p70^S6k is not affected by 24-h serum starvation, serum stimulation, or rapamycin treatment.