Table 1.
Two-hybrid analysis of p70 and neurabin
| Binding domain fusion protein (pPC97) | Activation domain fusion protein (pPC86) | Interaction assessed by β-gal filter assay | Interaction assessed by histidine prototrophy |
|---|---|---|---|
| p70 (amino acids 332–502) | neurabin (amino acids 486–751) | + | + |
| neurabin (amino acids 486–751) | p70 (amino acids 332–502) | + | + |
| p70 (amino acids 332–502) | cyclophilin | − | − |
| cyclophilin | neurabin (amino acids 486–751) | − | − |
| p70 (amino acids 332–502) | neurabin-GLAA (amino acids 486–751) | − | − |
| p70 Δ5 (amino acids 332–497) | neurabin (amino acids 486–751) | − | − |