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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Dev Biol. 2007 May 1;308(1):15–29. doi: 10.1016/j.ydbio.2007.04.031

Fig. 7.

Fig. 7

Decreasing FGFR1 or FGFR2 expression with siRNA decreases both branching and Lama5 expression but does not decrease Lama1 expression. A. Light micrographs of E12 SMGs cultured for 48 hours with either a scrambled control siRNA, siRNA to FGFR1 and FGFR2, or a combination of both FGFR siRNA. The decrease in branching is quantitated by counting the number of end buds after 48 hours of siRNA transfection. Knockdown of both FGFRs inhibits branching more than either alone. ANOVA ** P<0.01 compared to control. B. The knockdown of either FGFR1 or FGFR2 results in decreased Lama5 and increased FGF1 expression, but does not affect Lama1 expression. The knockdown of one epithelial FGFR also decreases the expression of the other by 48 hours, suggesting each receptor regulates the expression of the other. The gene expression was measured by real-time PCR, and the results are expressed in fold increase in gene expression compared to glands transfected with a nonsilencing control siRNA. P values are unpaired T-tests compared to 29S controls **P< 0.01, *P< 0.05.