Figure 3.

Explanted rd mice retinas cultured for 1 week in DMEM alone (a and b) or in the presence of dissociated young retinal cells (c and d), then double immunolabeled with PNA lectin (a and c) and arrestin antiserum (b and d). The same fields are shown in a and b and in c and d and reveal that PNA lectin labels rounded matrix-associated structures protruding from the retinal surface whereas arrestin is present throughout the photoreceptor cell bodies and outer segments. The differing focal plane of the two labels makes superimposition difficult, but the overlapping nature of the staining pattern can be observed (corresponding arrowheads in paired fields a and b and in paired fields c and d). (Bar = 10 μm.)