Figure 7.

Effects of lithium, PDC, or dihydrokainate on glutamate uptake and extracellular glutamate. Mouse cerebrocortical slices were prepared and restored as described in Experimental Procedures and then preincubated (average protein = 1.53 mg) in 3.2 ml of KHBS for 30 min with 5 or 10 mM LiCl, 1.25 or 2.5 μM PDC, or 15 or 30 μM dihydrokainate. Samples then were taken for enzymatic determination of medium glutamate, and the tissue was assayed for initial uptake of glutamate (5 μM) as described in Experimental Procedures. Shown is the average of three experiments with quadruplicate determinations in each experiment.