A single application of the 5-HT2 receptor agonist α-Me-5-HT did not significantly alter the frequency of spontaneous or respiratory-related GABAergic IPSCs in CVNs; a typical example is shown in A, and the summary data (n = 10 CVNs) in B. In contrast, multiple applications of α-Me-5HT evoked a significant decrease in the frequency of spontaneous (p < 0.01 n = 10) and fictive inspiratory-related (p < 0.001 n = 10) GABAergic IPSCs, that persisted for at least 1 hour, A, C. *** denotes p < 0.001 in this and all subsequent figures.