Figure 3.

Intracellular ROI accumulation of embedded parsley cells after local mechanical stimulation. Immediately after touching with a tungsten needle, cells were loaded with DCFH-DA for 7 min and subsequently irradiated with blue light for epifluorescence analysis. (A) Bright-field image; cell and needle in focal plane. (B) Blue light epifluorescence after 7 min of mechanical stimulation. (C) Bright-field image; unstimulated background cells of A in focal plane. (D) Blue light epifluorescence at the same time as shown in B. (E) Same as D but after 5 min of irradiation with blue light. n, nucleus. (Bar = 20 μm.)