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. Author manuscript; available in PMC: 2008 Oct 31.
Published in final edited form as: Eur J Pharmacol. 2007 Jun 29;572(2-3):94–101. doi: 10.1016/j.ejphar.2007.06.028

Figure 5.

Figure 5

Comparison of the antagonist action of 17PA on 5α-reduced steroid potentiation of muscimol-stimulated 36Cl- uptake in rat cortical synaptoneurosomes. a. 3α,5α-THP (3 μM) potentiation of muscimol-stimulated (1.5 μM) 36Cl- uptake relative to basal (P<0.0001, inset panel). Note that 17PA completely blocked 3α,5α-THP potentiation of muscimol-stimulated 36Cl- uptake (IC50 = 30.17 μM). b. Synthetic neurosteroid alphaxalone (3 μM) potentiation of muscimol-stimulated (1.5 μM) 36Cl- uptake (P<0.001, inset panel). Note that 17PA partially attenuated alphaxalone potentiation of muscimol stimulation (IC50 = 11.23 μM). c. 3α,5α-THDOC (1 μM) potentiation of muscimol-stimulated (1.5 μM) 36Cl- uptake (P<0.001, inset panel). 17PA partially attenuated 3α,5α-THDOC potentiation of muscimol-stimulated 36Cl- uptake (IC50 = 6.08 μM). d. pregnenolone sulfate inhibition of 3α,5α-THDOC potentiation (IC50 = 21.64 μM).