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. 2007 Aug 31;93(12):4462–4473. doi: 10.1529/biophysj.107.112821

FIGURE 5.

FIGURE 5

(a) Fluorescence intensities of the monomer (I3, 388 nm) and the excimer (IE, 475 nm) as a function of Py-met-chol mole fraction, in LUVs of DMPC. Fluorescence ratios I1/I3 (b) and IE/I3 (c) of Py-met-chol incorporated at 0.2, 0.5, or 2 mol % in LUVs composed of DMPC as a function of cholesterol mole fraction. Ratios are normalized taking ratio values for suspensions without cholesterol as the reference, which corresponded to I1/I3 and IE/I3 values of: 1.82 and 0.06 with 0.2 mol % of Py-met-chol, 1.89 and 0.15 with 0.5 mol %, and 1.99 and 0.27 with 2 mol %. Error bars represent standard deviations of two independent experiments. In all cases, the temperature was 37°C.