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. 2007 Aug 31;93(12):4462–4473. doi: 10.1529/biophysj.107.112821

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Copyright © 2007, Biophysical Society

This is an Open Access article distributed under the terms of the Creative Commons-Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/2.0/), which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fluorescence I_E/I₃ values of one set of various conditions (a) and normalized I₁/I₃ values for all experiments (b) involving Py-met-chol with living CHO-δ and CHO-wt cell suspensions. The arrow indicates the addition to the CHO-δ cell suspension of a solution of buffer (HEPES, n = 9), antagonist (Diprenorphin, n = 10), or agonist (Deltorphin II, n = 11), or addition of a solution of agonist to the CHO-wt cell suspension (n = 10). I₁/I₃ ratios are normalized taking ratio values measured before ligand addition. Error bars correspond to mean ± SE of n experiments. Agonist and antagonist were used at final concentrations of 1 μM. All measurements were carried out at 37°C. Lines are provided to facilitate reading.