FIGURE 5.

Dependence of Ba²⁺ trapping on the time mutant L273F spends in slow inactivation. Representative traces recorded without or with 10 mM Ba²⁺ that was applied for 12 s after 24 s (A) or 0.9 s (B) of depolarization to −20 mV, followed by washout (48 s). (C) Slow (open bars) and fast (solid bars) time constants of Ba²⁺ unblock after two different application times with (D) their respective slow (open bars) and fast (solid bars) amplitude components. Data shown are mean ± SE (n = 5; * p < 0.005).