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. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: Mech Dev. 2007 Jul 19;124(9-10):657–667. doi: 10.1016/j.mod.2007.07.005

Figure 1. MAPK and PI3K regulate mesoderm formation and gastrulation at different developmental stages in Xenopus.

Figure 1

Embryos were incubated with the MAPK inhibitor U0126 or the PI3K inhibitor LY294002 at 20μM (A) or 50μM (B) from stage 4 or stage 10 onward. DMZ explants were dissected at stage 10, mesodermal markers were analyzed by RT-PCR at stage 11, and the morphology of the explants was examined at stage 16. While both inhibitors reduced the elongation of the DMZ explants in a dose-dependent manner when applied from either early or late stages, only inhibitors applied from stage 4 could efficiently block expression of a panel of mesodermal markers.