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. 2007 Nov 30;3(11):e182. doi: 10.1371/journal.ppat.0030182

Figure 4. Primer Extensions on the Monomeric Circular and Linear HSVd and ASSVd RNAs.

Figure 4

(A and B) The cDNAs generated with the HSVd (−) primer PIII (A) and the ASSVd (−) primer PIV (B) were separated by denaturing PAGE. Lanes 1 to 4, sequencing ladders obtained with PIII and pBmHSVdE (A), and PIV and pBmASSVdS (B). Lanes 5, cDNAs from reverse transcription of ml HSVd (A) and ASSVd (B) (+) RNAs purified from transgenic A. thaliana expressing dt HSVd and ASSVd (+) RNAs, respectively. Lanes 6, cDNAs from reverse transcription of ml HSVd (A) and ASSVd (B) (+) RNAs purified from HSVd-infected cucumber and ASSVd-infected apple, respectively. Lanes 7, cDNAs from reverse transcription of mc HSVd (A) and ASSVd (B) (+) RNAs purified from HSVd-infected cucumber and ASSVd-infected apple, respectively. Portions of the HSVd and ASSVd (+) sequences are indicated on the left with asterisks marking the 5′-terminal nucleotide of the linear forms.

(C) Rod-like secondary structure predicted for HSVd and ASSVd, with the upper insets highlighting a portion thereof. Positions of the complementary primers PIII and PIV are indicated with arrows and bold fonts, and the processing sites with asterisks.