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. 2007 Nov 30;3(11):e185. doi: 10.1371/journal.ppat.0030185

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Heart-derived T cells were stimulated with T. cruzi Ag (25 μg/ml) for 18 h at 37 °C and were stained for CD4 or CD8, IFN-γ, and CD44 marker as described in Materials and Methods. Cells from B₂R^+/+ mice were gated on CD4⁺IFN-γ⁺ or CD8⁺IFN-γ⁺ lymphocytes and examined for expression of CD44. Dot plot profiles (n = 5 mice/group) are representative of results observed in two independent experiments. Column graphs (lower panel) indicate the mean ± SD of the frequency of IFN-γ-producing CD4⁺CD44⁺ or CD8⁺CD44⁺ T cells (n = 2). Statistics were done by ANOVA and pair-wise comparisons were done by the Tukey tests (*, p < 0.01).