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. 1989 Apr;171(4):2241–2243. doi: 10.1128/jb.171.4.2241-2243.1989

Use of gene replacement to construct Escherichia coli strains carrying mutations in two genes required for stability of multicopy plasmids.

H Flinn 1, M Burke 1, C J Stirling 1, D J Sherratt 1
PMCID: PMC209887  PMID: 2649493

Abstract

Escherichia coli mutants completely defective in ColE1 cer-mediated site-specific recombination have been mapped to two genes, xerA and xerB. In this study, xerA xerB double mutants were constructed by gene replacement with a lambda dv plasmid and were shown to be both viable and defective in ColE1 site-specific recombination.

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Selected References

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