Figure 1.

Immunoprecipitation of JHE with universal Hsp70 antiserum to quantify the extent of JHE-BP binding to modified forms of JHE. Larvae of M. sexta were injected with affinity-purified JHE or modified JHE, or the control samples BSA or CBE. Pericardial cells were removed 1 hr postinjection and homogenized in sample buffer. Aliquots of the homogenate were immunoprecipitated with the Hsp70 antiserum and Immunobead second antibody reagent. Data shown are for mean percent catalytic activity precipitated from three or four pericardial cell homogenates. (Inset) JH III is hydrolyzed to JH III acid by JHE of H. virescens with a k_cat/K_m that is indistinguishable from other JH homologs.