Table 1.
Kinetic parameters for wild-type and modified JHE
| Substrate | Enzyme | Km, μM | Vmax, μmol/min per mg | kcat, min−1 |
|---|---|---|---|---|
| [3H]JH III | JHE | 0.03 ± 0.01 | 1.57 ± 0.06 | 104 |
| JHE-29 | 0.05 ± 0.01 | 1.89 ± 0.05 | 125 | |
| JHE-524 | 0.06 ± 0.01 | 3.00 ± 0.02 | 198 | |
| JHE-KK | 0.02 ± 0.001 | 2.01 ± 0.02 | 133 | |
| HEXTAT* | JHE | 46 ± 6 | 55 ± 3 | 3,649 |
| JHE-29 | 49 ± 7 | 67 ± 4 | 4,466 | |
| JHE-524 | 77 ± 19 | 118 ± 14 | 7,800 | |
| JHE-KK | 59 ± 11 | 44 ± 3 | 2,904 |
Mean ± SD values are given for six replicates on a single recombinant protein preparation.
*
HEXTAT is S-(methyl) thiohexylthioethanoate, a colorimetric substrate.