Table 2.

Pharmacokinetic parameters^* for clearance of JHE following injection into the hemolymph of Manduca sexta

Enzyme	Rate constant of clearance,† hr−1	Mean t½‡ ±SD, min
JHE	1.28	37.3 ± 6.6
	0.98
	0.90
JHE-29	0.93	45.5 ± 1.2
	0.89
	1.15
JHE-524	1.34	34.2 ± 4.8
	0.89
	0.72
JHE-KK	1.51	46.2 ± 16
	1.05
	0.97

^*JHE was injected into hemolymph of larval M. sexta. Clearance of catalytic activity from the hemolymph was monitored up to 4 h postinjection by determination of the levels of JHE activity using JH III in a standard assay. 

^†Plasma concentration P at any time t (hr) is given by P = P₀e^−kt, where P₀ is the initial plasma concentration and k is the rate constant of clearance. 

^‡Data are presented for three experiments for each JHE type. Six larvae were bled to determine hemolymph activity at each of five time points for each experiment.