TABLE 2.
Kinetic and affinity constants for the interactions of TIAR123, TIAR12, and HuR12 proteins with U-rich and C-rich RNAsa
| Protein | RNA | Ka (1/M−1 s−1) | Kd (1/s) | KD (Kd/Ka [nM]) |
|---|---|---|---|---|
| HuR12 | U rich | (1.29 ± 0.01) × 108 | 4.35 ± 0.03 | 33.73 ± 0.50 |
| TIAR123 | U rich | (1.58 ± 0.02) × 106 | (1.56 ± 0.01) × 10−3 | 0.99 ± 0.02 |
| C rich | (2.72 ± 0.03) × 104 | (36.78 ± 0.12) × 10−4 | 135.2 ± 1.93 | |
| TIAR12 | U rich | (4.10 ± 0.11) × 106 | (2.83 ± 0.08) × 10−3 | 0.69 ± 0.04 |
| C rich | (3.48 ± 0.04) × 104 | (19.64 ± 0.06) × 10−4 | 56.39 ± 0.79 |
a
The association and dissociation rate constants (Ka and Kd) were determined as global fitting parameters for a 1:1 binding model. The equilibrium dissociation constant, KD, was determined as Kd/Ka.