FIG. 6.

Comparative RT-PCR analysis of NCAM- and polysialic acid-deficient mice. (Upper) Total RNA from forebrain was prepared at P0 from WT and mutant mice from the same litter, and expression levels of various genes were analyzed using specific primers by RT-PCR. Photographs of amplified fragments are shown. −, without reverse transcriptase; W1, W2, and WT are WT mice, P1 and P2 are II^−/−/IV^−/− mice, and NC are NCAM^−/− mice. (Lower) Amplified fragments were analyzed densitometrically and compared between WT and knockout mice in the same litter. At least two different mice of each genotype were used for statistical analysis. Error bars indicate standard deviations. *, P < 0.002; **, P < 0.005.