FIG. 5.
Dephosphorylation of BACH1 positively regulates G1 ATPase activity. FLAG-BACH1 cells were synchronized at G1/S with aphidicoiln and released into S phase, and at various times after release, cells were harvested followed by nuclear extract preparation and immunoprecipitation with M2-anti-FLAG antibody. The beads were treated with λ-protein phosphatase (+; lanes 4 to 6 and 10 to 12) or the heat-treated enzyme (−; lanes 1 to 3 and 7 to 9) and washed extensively, and the FLAG-eluted fractions from each time point were assayed for ATPase activity.