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. 2007 Jul 23;27(19):6818–6831. doi: 10.1128/MCB.00375-07

FIG. 1.

FIG. 1.

TRB3 expression is regulated during adipocyte differentiation. (A) TRB3 mRNA expression during adipocyte differentiation of 3T3-L1 cells. TRB3 mRNA expression was measured by quantitative real-time PCR and normalized to TBP mRNA expression. The data are represented as the percentage of maximal expression. The results are representative of three independent experiments. (B) TRB3 protein expression during adipocyte differentiation of 3T3-L1 cells. A total of 40 μg of total protein extracts was loaded, and adipocyte differentiation markers expression were assessed by Western blotting. For TRB3, 100 μg of protein was loaded and analyzed in a 12.5% polyacrylamide gradient gel and assessed by Western blotting. The results are representative of three independent experiments. (C) TRB3 mRNA regulation by the adipogenic cocktail components. 3T3-L1 cells were treated during 24 h with DEX, MIX, and insulin separately or in combination as described in Materials and Methods. TRB3 mRNA expression was measured by quantitative real-time PCR and normalized to TBP mRNA expression. The data are represented in relative units. The results are representative of three independent experiments. *, P < 0.01 compared to the “control (Cont) − Ins” value; #, P < 0.01 compared to the “control + Ins” value.