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. 2007 Jul 30;27(19):6794–6805. doi: 10.1128/MCB.01029-07

FIG. 2.

FIG. 2.

ZPBP proteins are intra-acrosomal proteins and present in amphibian, avian, and mammalian species. (A and B) Immunofluorescence microscopy showed different intra-acrosomal localizations of mouse ZPBP1 (red) and ZPBP2 proteins (green) in step 5 round spermatids (A) and in mature spermatozoa (B). (C) Domain structures of ZPBP proteins. The signal peptide was predicted by using the SignalP server (http://www.cbs.dtu.dk/services/SignalP) (14), and the immunoglobulin-like (IG-like) domain was predicted by using the SMART database (http://smart.embl-heidelberg.de) (23). Red bars represent the 15 conserved cysteines (Cys) among ZPBP proteins, and 8 of these Cys are located near the C terminus. (D) Protein sequence alignment shows the 15 conserved cysteines (red asterisks) outside of the signal peptide region as the defining feature of the ZPBP protein family. h, human; m, mouse; g, chicken (G. gallus); x, frog (X. laevis).