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. 2007 Jul 23;27(19):6647–6658. doi: 10.1128/MCB.00155-07

FIG. 1.

FIG. 1.

Downregulation of Rap1GAP in human thyroid cancer cell lines. (A) Rap1GAP expression was analyzed by Western blotting in a panel of human thyroid carcinoma cell lines (see Materials and Methods) and WRT cells. The same filter was probed for E-cadherin and vimentin. Equal protein loading was confirmed by blotting for actin. More than three experiments were performed, with similar results. (B) Rap1GAP mRNA was analyzed by RT-PCR. No DNA was added to the negative control (−), and WRT cells provided a positive control. Four experiments were performed, with similar results. (C) Phase-contrast images of human thyroid carcinoma and WRT cells.