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. 2007 Jul 23;27(19):6647–6658. doi: 10.1128/MCB.00155-07

FIG. 7.

FIG. 7.

Interference with Rap1 activity impairs Rac1 but not ERK activity in Ras-transformed cells. (A) Rac1 activity was assessed in Rap1GAP and LacZ virus-infected RasV12S35 cells. Rac1 activity in mock-infected cells (−) was set as 1. Results from three experiments are shown. (B) Rac1 activity was examined in Rap1N17 and LacZ virus-infected (MOI of 152) RasV12S35 cells. Rac1 activity in mock-infected cells was set as 1. Results from two experiments performed in triplicate (*, P < 0.05) are summarized. (C) RasV12S35 cells were pretreated with 10 μM UO126 (UO) for 30 min prior to wounding. Cells were imaged immediately and after 24 and 48 h. Three experiments were performed, with similar results. (D) Activating phosphorylation of ERK2 was compared in LacZ versus Rap1GAP virus-infected RasV12S35 (MOI of 6) and FTC-133 (MOI of 4) cells at 24 h postinfection. Equal protein loading was confirmed by Western blotting for AKT1. Three experiments were performed with RasV12S35 cells, and four were performed with FTC-133 cells, with similar results.