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. 2007 Jul 23;27(19):6933–6947. doi: 10.1128/MCB.02385-06

FIG. 8.

FIG. 8.

RA induced MUC5AC mRNA expression and secretion. NHTBE cells were grown in RA-deficient medium for 7 days and then further incubated with 1 μM RA for the indicated times (A and C) or with the indicated concentrations of RA for 24 h (B and D). MUC5AC mRNA expression and MUC5AC protein secretion were measured by QRT-PCR (A and B) and dot blot analysis (C and D) as described in Materials and Methods. Values shown are means ± SE of three independent experiments, each performed with triplicate cultures. *, P < 0.05; **, P < 0.01 (compared with RA-untreated control).