FIG. 10.

HOIL-1-mediated degradation of Bach1 in vivo. (A) Expression of HOIL-1 in wild-type (WT) and DN HOIL-1-expressing MEL cells were examined using anti-HOIL-1 antibody. The arrows indicate the positions of endogenous (Endo) and DN HOIL-1. The positions of molecular mass markers (in kilodaltons) are indicated to the left of the gel. (B) Control (wild type [WT]) and DN HOIL-1-expressing cells were cultured in the presence of 10 μg/ml cycloheximide (CHX) for 0 to 240 min. Total cell extracts were analyzed with antibodies against Bach1 and actin. (C) Determination of the degradation half-life of Bach1. The band intensities in panel A were measured by densitometric analysis. The relative mean values (Bach1/Actin) against CHX chase time from two independent experiments are shown; the average values are shown below the plot.