Figure 1.
Representative fluorescence images of the effect of ouabain on ICP8-GFP. The left column shows signal from the Hoechst 33258 DNA stain and the right column displays the same field in the GFP channel. The top two rows (panels A–D) show signal for Vero cells infected with ICP8-GFP at an MOI of 10 for 18 hours. Cells in the top row (panels A and B) were mock treated, while cells in the middle row (panels C and D) were treated with 14 μM ouabain. The bottom row (panels E and F) shows signal from mock infected cells.