Table 1.

Antiviral effect compared to inhibition of the sodium potassium ATPase

Compound	Drug concentration that reduces Na/K ATPase activity by 50%	Drug concentration that reduces viral yield by 90%
Ouabain	23 nM	40 nM
Bufalin	53 nM	15 nM
Digitoxin	78 nM	70 nM
Digoxin	120 nM	150 nM
Strophantidin	180 nM	500 nM
Ouabagenin	640 nM	2500 nM

Vero cells were infected with wild type HSV at an MOI of 10, and treated with a range of concentrations of the indicated inhibitors of the sodium potassium pump. Viral yield was determined by titration at 24 hours post infection. The right column contains the concentration of each drug that was sufficient to reduce viral yield by 90%. The middle column contains previously reported concentrations of drug that inhibited ATPase activity of cardiac membrane extracts by 50% (Pullen et al., 2004).