Table 3.
Strain/plasmid* | hupSp sequences (bp upstream of +1) | Nitrogenase† activity | % inhibition |
---|---|---|---|
UNF122 | None | 0.53 ± 0.15 | 0 |
UNF122 (pBC KS) | None | 0.56 ± 0.18 | 0 |
UNF122 (pBC315) | 880 | 0.06 ± 0.05 | 91 |
UNF122 (pBC47) | 173 | 0.06 ± 0.04 | 90 |
UNF122 (pBC58) | 88 | 0.54 ± 0.10 | 0 |
Inserts from plasmids pHL315, pHL47, and pHL58 were cloned in pBC KS to yield pBC315, pBC47, and pBC58, respectively.
Values [μmol of C2H2 reduced ⋅ h−1 ⋅ (ml of culture)−1] are the average of four independent determinations ± SE.