Figure 1. Immunization with a replication-defective HSV-2 virus prevents lesion development following an ocular challenge with HSV-1.
Groups of 6 to 8 CD-1 male mice were immunized with 104, 105 or 106 PFU of HSV-2 dl5-29 virus on days 0 and 21 via subcutaneous inoculation, and a group inoculated with PBS was included as a negative control. On day 35 all mice were challenged on the eye with HSV-1 KOS following corneal scarification. Mice were then monitored for lesion development for twenty days post-challenge.